BIOL 200 Lecture 5: BIOL 200 Lec 05
5 Oct 2017
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Document Summary
Polymerase ii transcribes mrnas and 4 small nuclear rnas (snrna) that take part in splicing & micro rnas (mirnas) involved in translation control. Polymerase iii transcribes trna, 55 rrna, and other small stable rnas including one involved in splicing. Proteins can be separated by chromatography: samples put in a gel matrix separate into proteins with the help of a solvent. The proteins bind to the material differently depending on their properties so bands will form from the separation. The one that comes out rst sticks to the material less and is ushed down faster. Bigger proteins run through column faster because they can go through the sides & the small ones get caught in the middle with all the other beads. Gel bead is positively charged so proteins with positive charges will go through the column faster and negative proteins will interact with the positive beads & go through slower.
