BIOL 200 Lecture Notes - Lecture 24: Wild Type, Coiled Coil, Sh3 Domain
25 Jan 2017
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Document Summary
Dominant negative allele: produces a mutant form of the protein that inhibits endogenous function; has inhibitory biochemical function that inhibits many/all gene of a family. Dsrna is chopped by dicers into fragments, which are used by a machinery to identify and estroy similar rna. Protein structure: primary (sequence) secondary (local folding) tertiary (long-range folding) quaternary (multimeric organization) supramolecular (large-scale assemblies) Protein function depends on amino acid sequence: aa sequence contains all the info to give 3d structure and function of protein; primary sequence is direction (has n-terminus and c-terminus); r groups are the differences between amino acids. Proteins = natural polypeptide or a complex of polypeptides with a well-defined structure: reminder: one polypeptide = one rna translation. Secondary structure random coil: no intramolecular noncovalent interaction between amino acids. Stabilized structures: polypeptides are stabilized by noncovalent interactions, usually hydrogen bonds. Alpha helix and beta sheet: together make up to 60% of polypeptides; also u-turns.
