BIOL 200 Lecture Notes - Lecture 17: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Restriction Fragment Length Polymorphism

• School: McGill University
• Department: Biology
• Course: BIOL 200
• Professor: Richard Roy

Document Summary

Lecture 17 molecular genetics methods ii: coli and bacteriophage lambda are the workhorses of molecular biology. Most higher organisms use similar processes as bacteria and viruses (phage) as to replicate, transcribe, and translate their essential cellular molecules. Purified components (i. e. enzymes) facilitated the rapid progress. Paul berg and others were the first to successfully introduce exogenous dna fragments into bacteria to produce a recombinant gene product. This milestone ushered in the era of recombinant dna technology. Clone: group of organisms produced from one stock or ancestor, one such organism, person or thing regarded as identical to another. It can be used in different circumstances to point out different procedures: early frog embryo. One single blastomere can develop more than one: cloning by: replacing the nucleus of an egg cell by the nucleus of an adult, somatic cell, implanting a resulting embryo.