BIOL 200 Lecture Notes - Lecture 8: Plasmid, Ecori, Ampicillin
3 Oct 2016
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Some processes are more powerful at being able to recruit rna pol ii: genes that are transcribed at high levels have strong promoters like tata box starting 35 bp upstream of the start site. Decresing or mutating tata will decrease the promoter strength dramatically. If you put in extra sequences over the 35 bp or remove (distance), it will be too close or too far in mammals. Affect ability of tata box to work: some genes have an initiator element at the -1 and an a at the +1 positions. No good consensus sequence has been defined. Bre element: tfiib orient more effectively. Inr: part of it is transcribed, lead t othe discovery of dpe. Dpe (downstream promoter element: in the absence of tata, this transcribes) Transcription initiation by rna pol ii requires gtfs position the active site of. Tfiid (tbf + 13 tafs), tfiia, tfiib, tfiif and pol ii (preformed complex)= _closed complex: polii positioned over transcription site.
