BIOL 200 Lecture Notes - Lecture 7: Immunoprecipitation, Consensus Sequence, Noncoding Dna
3 Oct 2016
School
Department
Course
Professor
Document Summary
Liquid chromatography can be used to sequentially purify a specific protein from a complex mixture. Eukaryotes have 3 rna o distinguished by differential sensitivity to alpha-amanatin. Pol i is insensitive to 1 ug/ml and 10 ug/ml. Pol iiii is insensitive to 1ug/ml but sensitive to 10 ug/ml. Eukaryotes have 3 rna polymerases containing subunits that have homology to bacterial rnap o o. Beta and beta" form core complex find transcription size. W is stabilizing thing that"s holds it together. Rna pol ii has a carboxyl terminal domain (ctd) that is phosphorylated in actively transcribing polymerases. Rna pol ii transcription initiation: tbp binds to dna, tata box binding protein. Binds in the minor groove: tbp is part of tfiid, a complex of tbp and. Major mechanism for controlling production of the protein encoded by a given gene. Transcription of a gene can be: repressed. Little or no mrna is synthesized: activated. Up to 1000x or more mrna is synthesized.
