BIOL 200 Lecture Notes - Lecture 10: Orf 2, Intron, Non-Coding Rna
Document Summary
The infested genome: ltr retrotransposons, lines and sines. Two loci were involved in breakage: the dissociation locus (ds) at breakpoint, the activator (ac, which mcclinctock was unable to map. With the breaks you lose genes (some code for colour) causes the expression of the recessive gene. In other strands some phenotypes reverted: proposed that ac and ds were mobile. Cut and paste: dna transposons, transposition. Types: ltr-retrotransposons (long terminal repeats, lines (long interspersed nuclear elements, sines (short interspersed nuclear elements, processed pseudogenes. Disrupts the central dogma: reverse transcription where rna and become dna. Causes host cell to make retrovirus proteins. Process: genomic ssrna has reverse transcriptase (protein, retrovirus is engulfed into a cell. Retroviruses is surrounded by nucleocapsid: the nucleocapsid is broken down by the cell and then reverse transcription occurs. Single stranded rna is transcripted into double stranded dna: dna is integrated into host cell genome (transgenic, host cell transcribes viral proteins into the host cell.
