BIOL 115 Lecture Notes - Lecture 9: Nuclear Membrane, Kinetochore, Metaphase

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25 Jun 2016
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G2: replication of the centrosome (and centrioles in some cases) Spindle forms: chromosome condensation chromatids (1dna) become evident, kinetochores form. Prometaphase: nuclear envelope breakdown so microtubules have access. Polar microtubules and kinetochore microtubules form (form spindle network: chromosomes arrive on metaphase plate. Metaphase: chromosomes are lined up on the metaphase plate as the microtubules from each pole grab" chromosomes near centromere, and pull to align on metaphase plate. Sister chromatids bound to kinetochore microtubules on opposite spindles (each chromatid bound to a difference centrosome) Anaphase: centromeres separate, kinetochore microtubules shorten (pulled to opposite poles) Spindle breaks down: chromosomes decondense, nucleus reforms. Cytokinesis: cells divide (optional, animals purse string mechanism actin and myosin constrict like a purse string and divide the cell. Plants vesicles fuse to make cell membrane and cell plate = new cell wall dividing mother cell. This is optional , eg muscle cells have many nuclei (are syncytial) as they undergo mitosis without cytokinesis.

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