BIOL 112 Lecture 21: BIOL 112 – Lecture 21 – Biotechnology II

• School: McGill University
• Department: Biology
• Course: BIOL 112
• Professor: Frieder Schoeck

Document Summary

Biol 112 lecture 22 biotechnology ii. Library screening o: radioactive genes pair with only the pair of genes with insulin. Making bacterium recognized the insulin gene as a gene o: to express it, use expression vector. However, problems arise: what is needed to happen, what you actually get, research method. Cloning an unknown gene: why clone: to understand what the gene encodes and how it works, ex. Determine the biochemical basis of the inherited disease cystic fibrosis. Cystic fibrosis: characterized by respiratory infections, digestive problems and sterility, autosomal recessive trait, occurs in about 1/2500 caucasian newborns. How do you find a gene if you do not know what protein it makes: map it o. Problems with mapping: there are not many phenotypes in humans exhibiting simple mendelian inheritance for you to map against, nor can you control human mating. Polymorphic bamhi sites are distributed throughout genome o.