BIOL 112 Lecture : BIOL 112 Labs 1-12.doc

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17 Mar 2012

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BIOL 112 Full Course Notes

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Lab coats and safety goggles with side shields should be worn in the lab at all times. Material safety data sheets (msds) available for chemicals used in lab exercises. Headings (probably don"t need to know this, but just in case): product information, information on the preparation of the msds, hazardous ingredients, physical data, fire and explosion hazards, reactivity data, toxicological properties, preventive measures, first aid measures. Classes (same as the above): compressed gas, flammable and combustible material, oxidizing material, poisonous and infectious material (divisions 1 (immediate toxic effects), 2 (other toxic effects), and 3 (biohazardous infectious materials)), corrosive material, and dangerously reactive material. Allows us to see at the level of micrometres instead of millimetres. Microscopes are expensive instruments, so don"t, say, use them as makeshift baseball bats. They aren"t really suited for hitting home runs, anyway. The one we use in this lab is called the zeiss compound microscope.

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LAB MODULE: BASIC MICROSCOPY â STUDY HELP

The information in this document is helpful in preparing for the Basic Microscopy Pre-Lab Quiz on Canvas and the Basic Microscopy quiz on Late Nite Labs.

Instructions:

â¢ Go to your Late Nite Labs course site and click on the Basic Microscopy link. The lab manual content will appear on the right side of the screen.

â¢ PRINT and READ all of the information found in the Basic Microscopy lab manual.

â¢ Use the information to answer the following questions.

Questions

â¢ Fill in the remainder of the chart.

Objective Lens	Objective Magnification	Ocular Magnification	Total Magnification
Scanning	4X	10X	40X
Low Power	10X	10X
High Power	40X	10X
Oil Immersion	100X	10X

â¢ Do all organisms consist of more than one cell?

â¢ What is the structural and functional unit of living things? In other words, what is considered the basic building block of LIFE?

â¢ What three structures are found in the cells of ALL organisms, both prokaryotic and eukaryotic? You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all three structures. Note: plasma membrane = cell membrane

â¢ List the major differences in prokaryotic cells and eukaryotic cells? Include the location of genetic material in a eukaryotic versus prokaryotic cell.

â¢ List the organelles found in a eukaryotic cell that are not found in a prokaryotic cell. You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all of these.

â¢ In eukaryotic cells, genetic information is found in the form of DNA. Is this true for prokaryotic cells?

â¢ Are flagella and cell walls unique to eukaryotes? Why or why not?

â¢ The __________is the part of a cell that allows the movement of substances into and out of the cell and protects the cell from its environment.

â¢ The__________is a substance that contains all of the dissolved and suspended ions and other molecules needed to keep the cell alive.

â¢ A__________is an organelle that serves as the site of photosynthesis in a eukaryotic cell.

â¢ Some organisms have cells that contain a__________that gives the cell a more structured shape and strengthens the cell by osmotic pressure.

â¢ Describe dark field microscopy.

â¢ Describe phase contrast microscopy.

â¢ Why do scientists use dark field microscopy and phase contrast microscopy when studying prokaryotic cells?

â¢ How does phase contrast microscopy help scientists visualize difficult specimens?

â¢ Fill in the remainder of the chart. The organisms studied during this lab are: Amoeba, Spirogyra, cardiac muscle, and bacteria.

Structure	Function	Organisms Studied
Plasma Membrane		Amoeba, Spirogyra, cardiac muscle, and bacteria
Cytoplasm
Nucleus	Contains the genetic material of a eukaryotic cell
Chloroplast		Spirogyra
Cell wall

Please explain in great detail(while mainlyFOCUSING ONTHE PHYSIOLOGICAL PATHWAYS),the physiology involved with the way each of theseeffects(Including effects Immediately after exercise, apnea, andcooling. Also including the effects several minutes later)Heart rate, AND BloodPressure:

**also, please include any and all information that isnecessary to understand the material you discuss in youranswer**

Exercising (quickly walking up and down a stair case for 5minutes)
Apnea (holding one's breath as long as they can)
Cold temperatures (by placing an icepack on one's arm for 10minutes. (mention effects during the 10 minute cooling period, andthe effects for about 5 minutes after the icepack is removed))

cerisecockroach684

Material Science

Question 1

Muscle fibers slide smoothly across each other in both directions.

True

False

Question 2

Cell membranes are composed of single layers of fat molecules.

True

False

Question 3

In mammals, the percentage composition of fats and minerals is roughly the same.

True

False

Question 4

Both RNA and DNA have a double helix structure.

True

False

Question 5

The primary constituents of muscles are

	a)	the proteins kerotin and actin
	b)	the proteins lycine and actin
	c)	the proteins actin and liposine
	d)	the proteins glycine and lycine
	e)	the proteins myocin and actin

Question 6

Hydroxyapatite is a component of endoskeletons.

True

False

Question 7

There are thousands of types of amino acids.

True

False

Question 8

Amorphous silicon is a component of some biomaterials.

True

False

Question 9

The biosynthesis of both collagen and polysaccharides starts in the cell nucleus.

True

False

Question 10

After going through alkyline hydrolysis, the remaining solids are primarily hydroxyapatite.

True

False

Question 11

Calcium carbonate is a mineral found in some biomaterials.

True

False

Question 12

Proteins, chitin, and cellulose are build up from proteins

True

False

Question 13

There are thousands of types of proteins.

True

False

Question 14

Chitin is the most abundant natural polymer.

True

False

Question 15

Keratin is formed from a structure of living cells.

True

False

Question 16

Some biopolymers are hard.

True

False

Question 17

Polymeric biomaterials are comprised of collagen and elastin.

True

False

Question 18

Cell membranes are made of double layers of proteins.

True

False

Question 19

Which is NOT an example of a hard biopolymer?

		a)Eggs
		b)Insect Shells
		c)Hooves
		d)Nails
		e)Cellulose

Question 20

Polysaccharides are built up hierarchically from amino acids.

True

False

Question 21

Unit cells have faces that are

	a)	rhombus
	b)	square
	c)	rectangular
	d)	parallelegrams
	e)	circular

Question 22

Unit cells are

		a)the building blocks of crystalline material
		b)the building blocks of DNA
		c)the building blocks of polymers
		d)the building blocks of amorphous materials
		e)the building blocks of biomaterials

Question 23

Which radioactive semi-metal is the only element with a Simple-Cubic (SC) structure?

		a)Polonium
		b)Uranium
		c)Radium
		d)Plutonium
		e)Dilthium

Question 24

The common crystal structures in metal are

		a)FCC, BCC, HCP
		b)FCC, BCC, HCP, and SC
		c)HCC, BCC, FCP
		d)HCC, BCC, FCP and SC
		e)FCC, SEC, BCC

Question 25

Diffraction results in

		a)constructive interference
		b)delamination of the planes
		c)reverse polarization
		d)altered planar spacing
		e)destructive interference

Question 26

Unit cell indexing schemes provide

		a)location information for grain boundaries
		b)descriptions of the atoms, planes, and axis directions
		c)diffraction indexes for the crystal material
		d)the location of the unit cell in the larger crystalline matrix
		e)the packing arrangement

Question 27

Hexagonal crystal structures cannot be close-packed.

True

False

Question 28

To determine crystal structure and spacing, scientists use

		a)magnetic resonance imaging
		b)X-ray diffraction
		c)optical microscopes
		d)scanning electron microscopes
		e)atomic force microscopes

Question 29

A scanning probe (aka atomic force) microscope can be used to image and manipulate individual atoms.

True

False

Question 30

Crystal planar density is taken as

		a)the number of atoms per unit area for a plane
		b)the planar stacking order
		c)the number of atoms that are closely packed
		d)the number of equivalent atoms per unit cell
		e)the number of atoms per unit length for an axis

Question 31

The atomic packing factor represents

		a)the mass ratio of the unit cell
		b)the number of nearest-neighbor atoms
		c)the fraction of solid sphere volume in the unit cell
		d)the unit cell edge length (a) divided by the atomic radius (R)
		e)the density of the material

Question 32

When an elemental solid can have more than one crystal structure, it is said to have

		a)allotropy
		b)polymorphism
		c)polytropy
		d)elemorphism
		e)anthropomorphis

Question 33

The crystallographic linear density

		a)is determined by the atomic packing factor
		b)is six for HCP unit cells
		c)is five for BCP unit cells
		d)is determined by the coordination number
		e)is how many atoms lie along a unit length in that direction

Question 34

Bragg's law specifies

		a)the condition for when electrons will be refracted upon striking a crystal
		b)the condition for when light will be polarized upon striking a crystal
		c)the condition for when x-rays will be absorbed upon striking a crystal
		d)the condition for when x-rays will be refracted upon striking a crystal
		e)the condition for when light will be refracted upon striking a crystal

Question 35

Two features of a crystal unit cell structure are

		a)atomic number and elemental packing factor
		b)the number of interstitial and host atoms
		c)coordination number and atomic packing factor
		d)the number of faces and the number of atoms
		e)the number of substitutional and interstitial atoms

Question 36

Imperfections in semiconductors are used to create transistors and other electronic devices.

True

False

Question 37

The majority of crystalline solids are single crystals

True

False

Question 38

The grain structure of a polycrystalline material can be observed in the material without significant preparation.

True

False

Question 39

There are three types of electron microscopes, Transmission (TEM), Scanning (SEM), and Tunneling (TUEM).

True

False

Question 40

Defects are important for solid catalysts

True

False

Question 41

The mean intercept length is a measure of grain diameter.

True

False

Question 42

Imperfections in materials do not generally change their properties.

True

False

Question 43

The mean intercept length is computed from the average length of a grain boundary edge.

True

False

Question 44

Catalysts chemically combine with the reactant molecules in order to speed up the reaction.

True

False

Question 45

For a particular material, the higher the temperature the greater the number of vacancies.

True

False

Question 46

A twin boundary is a linear defect where the screw angles on each side of the boundary are equal.

True

False

Question 47

The regular pattern of crystal order is disrupted at a grain boundary.

True

False

Question 48

Solid solutions can only be formed by liquid material cooling to a solid.

True

False

Question 49

Alloys do not usually have significantly difference properties of the constituent metals.

True

False

Question 50

When an atom is missing from a crystal structure position, it is referred to as a self-interstitial.

True

False

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BIOL 112 Lecture : BIOL 112 Labs 1-12.doc

BIOL 112 Full Course Notes

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