BIOL 112 Lecture 16: Lecture 16 - DNA Discovery and Replication.pdf

• School: McGill University
• Department: Biology
• Course: BIOL 112
• Professor: Frieder Schoeck

Document Summary

Purifying a gene: : a method of isolating cell components, an assay to determine if it is a gene! Testing the subcomponent that has been separated out : grind up an organism , extract lipids and proteins with organic solvent (ie phenol) , precipitate with ethanol ! An assay is way of measuring something ! Can measure a substance (ex. assay for starch) ! Can measure an abstract phenomenon (ex. assay for memory -> mice in a maze) ! Extract lipids and proteins with organic solvents (ex. phenol) ! Nuclei ground up, lipids and proteins are extracted using phenol ! Nucleic acids are precipitated with ethanol ! Used 2 strains of streptococcus pneumonia ! Smoothness/ roughness was an inheritable trait ! R-strain was transformed by s-strain and killed mice ! Conclusion: a chemical component from one cell is capable of genetically transforming. Remaining question: what component of cell was responsible for transformation? another cell !