BIOL 2030 Lecture Notes - Lecture 15: Stem-Loop, Non-Coding Rna, Heterochromatin

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indigobat594

26 Feb 2017

School

Dalhousie University

Department

Biology

Course

BIOL 2030

Professor

Herbinger Christopher

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Document Summary

Lecture 15 - transcription (end), rna interference (rnai) and gene editing. Transcription ends after a terminator sequence is transcribed. Two major types of terminators in bacteria: rho-dependent (requires rho protein) Rho binds to rna upstream of the terminator at the rho utilization (rut) site. Rna polymerase pauses when it reaches the terminator sequence and rho catches up. Rho unwinds dna-rna hybrid using helicase activity: rho-independent. Promoters used by rna polymerase ii: transcribes pre-mrna, which is processed into mrna, promotor = core promoter + regulatory promoter. Located upstream of core promoter - exact location can vary. Transcriptional activator proteins bind to consensus sequences and affect the rate of transcription. Minimal sequence required for accurate transcription initiation. Includes a number of consensus sequences for transcription factor binding. Basal transcription apparatus: consists of rna polymerase ii, general transcription factors (tf), and mediator protein (rna.

Related Questions

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

BIOL 2030 Lecture Notes - Lecture 15: Stem-Loop, Non-Coding Rna, Heterochromatin

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