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BIOC 3300 Lecture Notes - Lecture 16: Dynamin, Facilitated Diffusion, Phosphatidylcholine

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coffeeoyster95
4 Apr 2016
School
Dalhousie University
Department
Biochem & Molecular Biology
Course
BIOC 3300
Professor
Mc Leod Roger

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Document Summary

Cylindrical (no curvature) - similar sized headgroup and tail. Cone (negative curvature) - small headgroup relative to large tail. Atp-dependent phospholipid translocases: what catalyzes the transverse flip-flop of phospholipids between er leaflets, and do they require energy? flippases. Er: what lipid has a relatively phosphatidylcholine similar concentration in every cell membrane, which membrane has the. Coat proteins polymerize on cytosolic side of donor membrane, binding to adaptor proteins. Dynamin is hydrolyzed to gdp, cuts vesicle off the donor membrane. Arf-gdp (inactive) has n-terminal helix that anchors it to membrane: what are the active and inactive forms of. Arf: what are features of the active form of arf, what lipid does phosphoinositols. Ap bind to in the membrane: what is the shape triskelion of clathrin, what are the features of intermembrane transport, what snare is on the vesicle and target organelle? v-snare (vesicle) t-snare (organelle, what is the function of. Snares? recognize and tether vesicles to membranes: what are rab-

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Related Questions

True or False? True False
1. Proteins rapidly pass artificial membrane (liposome).--------------------------------------------------------------------------------------( ) ( )
2. Amphiphatic detergent such as SDS spontaneously forms bilayer inwater. ------------------------------------------------------------( ) ( )
3. Cholesterol does have a hydrophilic head and hydrophobic tail.-------------------------------------------------------------------------( ) ( )
4. Fat molecules are one of the major components of the plasmamembrane.------------------------------------------------------------- ( ) ()
5. Phospholipid molecules such as phosphatidylcholine spontaneouslyform bilayer. ----------------------------------------------------( ) ( )
6. Flippase facilitates lateral movement of phospholipids withinthe plane of the membrane.----------------------------------------- ( ) ( )
7. Glycolipids make bilayer less permeable to small water solublemolecules.----------------------------------------------------------- ( ) ()
8. Membrane inner leaflet is enriched with phosphatidylcholine,Sphingomyelin, and Glycolipid. -----------------------------------( ) ( )
9. Plasma membrane proteins can have an enzyme activity.--------------------------------------------------------------------------------( ) ( )
10. A single pass membrane protein must have an amphiphatic alphahelix.-------------------------------------------------------------- ( )( )
11. Plasma membrane proteins can be linked to a lipid molecule atthe outer leaflet of the plasma membrane. --------------------- ( )( )
12. The lateral motility of membrane proteins can be restricted bytight junctions.------------------------------------------------------ ( ) ()
13. Channel proteins have specific binding sites for ions and thusslower than carrier proteins.--------------------------------------- ( ) ( )
14. An antiport would function as a symport if its orientation inthe membrane were reversed. ---------------------------------------( ) ( )
15. A transmembrane pore can be formed by multiple amphipathicalpha helices. ----------------------------------------- ( ) ()
16. Porin proteins form water-filled channels in the outer membraneof a bacterium and are mostly composed of beta sheets. ---- ( ) ()
17. Glucose uniporters localize at the apical side of theintestinal epithelium.------------------------------------------------------------ ( ) ()
18. GAP junction is a selective pore for anions.-----------------------------------------------------------------------------------------------( ) ( )
19. A voltage-gated Na+ channel adopts only two conformationstates: open and closed.----------------------------------------------- ( ) ( )
20. Voltage gated K+ channel opens faster than the Voltage gatedNa+ channels.-------------------------------------------------------- ( ) ()
21. Opening of chloride channel at the synapse will help to fire anaction potential.------------------------------------------------------ ( ) ()
22. The nuclear pore complex forms a gate through which moleculespass the nucleus uni-directionally. -------------------- ( ) ()
23. A completely folded protein is imported into a mitochondrionthrough a large non-selective pore.--------------------------------- ( ) ( )
24. ER signal sequence contains multiple lysine and arginineresidues.----------------------------------------------------------------------( ) ( )
25. The plasma membrane is a sorting station of inward endocyticpathway.---------------------------------------------------------------- () ( )
26. The trans Golgi functions as a sorting station of outwardsecretory pathway.----------------------------------------------------------- ( ) ()
27. Vesicles budding from ER are coated with Clathrin.----------------------------------------------------------------------------------------( ) ( )
28. RabGTPases and tethering proteins are the major specificitydeterminant of vesicle transport system. --------------------------( ) ( )
29. v-SNARE proteins are one of the tethering proteins.------------------------------------------------------------------------------------( ) ( )
30. In secretory cells, the constitutive and regulatory pathways ofexocytosis diverge at the cis Golgi network. ------------------ ( )( )
31. Many proteins are posttranslationally glycosylated at tyrosineresidues in the ER.------------------------------------------------- ( ) ( )
32. Cells import LDL molecules exclusively through pinocytosis.-------------------------------------------------------------------------( ) ( )
33. Cells recycle LDL receptors from lysosome to the plasmamembrane.----------------------------------------------------------------- () ( )
34. Phagosomes fuse with late endosome.------------------------------------------------------------------------------------------------------( ) ( )
35. Small vesicles from ER near the plasma membrane can fuse withthe plasma membrane upon bypassing Golgi apparatus. -- ( ) ()
36. If a Sec mutant showed an accumulation of newly synthesizedproteins at ER,
then the Sec gene is likely a component of vesicle-buddingmachinery of trans-Golgi network.------------------------------------ ( ) ( )
37. A signal recognition particle is a single pass trans-membraneprotein of the ER membrane.---------------------------------------- ( ) ( )
38. Typical animals cells display higher levels of intracellularsodium than extracellular compartments.------------------------------ ( ) ( )
39. Typical animals cells display higher levels of intracellularchloride than extracellular compartments.----------------------------- ( ) ( )
40. The Na-K Pump is a GTPase that can adopt active and inactiveconformational states.----------------------------------------------- ( ) ( )
1. Phospholipase
C-­-ß
produces
phosphatidylinositol
3,4,5-­-trisphosphate
and
diacylglycerol.
(
)
(
)
2. PDGF
activates
GPCR.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
3. Rho
is
an
example
of
heterotrimeric
G
proteins.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­-
(
)
(
)
4. All
members
of
the
G
protein
coupled
receptors
interact
with
small
GTPase
Ras.
-­--­--­--­--­--­--­--­--­--­-
(
)
(
)
5. Adrenaline
is
an
example
of
the
ligands
for
the
RPTK
family.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
6. Ras
GEF
activates
RPTK.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
7. PH
domain
containing
proteins
bind
to
activated
EGFR.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
8. Ligands
for
GPCR
proteins
induce
dimerization
of
the
receptor.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
9. Grb2
is
an
adaptor
protein
that
binds
to
a
phospho-­-tyrosine
residue
of
activated
RPTK
through
a
PH
domain.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
10. When
a
cell
is
expressing
a
large
number
of
dominant
negative
EGFR
proteins,
an
introduction
of
a
constitutively
active
Ras
mutant
will
allow
EGF
signaling.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
11. PI3K
generates
phosphatidylinositol
3,4,5-­-trisphosphate
from
IP3.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
12. Calcium
inhibits
PKC.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
13. cAMP
activates
PKA.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
14. The
MAP
kinase
ERK
inhibits
MAP
kinase
kinases.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­-
(
)
(
)
15. cAMP
is
an
example
of
a
first
messenger.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­----­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
16. All
7-­-Transmembrane
receptors
are
G
Protein
Coupled
Receptors.
-­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
17. Both
Ga
and
Gß
subunits
of
the
heterotrimeric
G
protein
can
mediate
GPCR
signaling.
-­--­--­-
(
)
(
)
18. Both
Ga
and
Gß
subunits
of
the
heterotrimeric
G
protein
have
GTPase
activity.
-­--­--­--­--­--­--­--­--­--­--­--­-
(
)
(
)
19. The
Gas
subunit
of
the
heterotrimeric
G
protein
suppresses
the
function
of
Adenylyl
cylase.
(
)
(

I have this questions for my homework i need help with them look please it is important i need help so please give me the correct answers for each question and also please be clear to understand and aslo be correct it is very important becuase i need them so i really need the right answers. please help me and on Questions 6 and 9 please help me with it hope you can help me with the correct answers please

Question 5

At a constant temperature, if you increase the number of unsaturated fatty acid chains in the phospholipid, the membrane will become......

less fluid
more fluid
fluidity will be unchanged

Question 6

Bacteria in a hot spring at 80 degrees celsius would be expected to have ______________ in their cell membrane

high proportions of saturated phospholipids
high proportions of unsaturated phospholipids
an equal ratio of saturated to unsaturated phospholipid

Question 7

Membrane proteins have an important role in membrane fluidity

True
False

Question 8

The HIV virus can't attach to cells that have a mutation in the CCR5 gene and so such individuals are resistant to HIV. What population of people have a higher incidence of the mutant CCR5 gene and why?

europeans because their ancestors survived the bubonic plague
North Americans because their ancestors survived small pox
europeans because they have a longer evolutionary past with HIV
Africans because they have a longer evolutionary past with HIV

Question 9

Nematode trapping fungi have ______________ molecules called ______________ that recognize specific carbohydrates on the surface of the nematode worms. This is how a fungus can recognize that its prey, the nematode worm, has wriggled into the loop. Write two words on lowercase separated by a space with no other syntax

Question 10

Diffusion requires energy

True
False

Question 11

The net direction of molecule movement when molecules diffuse is

down their concentration gradient
random directions

Question 12

In osmosis, water molecules from a solution of higher solute concentration cross a semi-permeable membrane into a solution of lower solute concentration?

True
False

Question 13

Which best defines osmosis?

In osmosis, water from a solution of lower solute concentration move across a semi-permeable membrane into a solution of higher solute concentration?
In osmosis, water from a solution of higher solute concentration move across a semi-permeable membrane into a solution of lower solute concentration?

Question 14

Animal cells placed into a hypotonic solution would most likely?

lyse (burst)
shrink
remain unchanged in size

Question 15

Glucose and amino acids move into cells via carrier proteins, the same protein can move glucose or amino acids

True
False

Question 16

ATP powered pumps move substances into and out of cells by diffusion?

True
False

Question 17

For every 3 Na ions moved out, 2 K ions are moved into a cell by one cycle of the sodium-potassium pump.

True
False

Question 18

Bulk transport of fluid and any of the substances within that fluid is called

pinocytosis
phagocytosis
receptor-mediated endocytosis
exocytosis

I have this questions for my homework i need help with them look please it is important i need help so please give me the correct answers for each question and also please be clear to understand and aslo be correct it is very important becuase i need them so i really need the right answers. please help me and be correct and clear it is important ok

Question 5

At a constant temperature, if you increase the number of unsaturated fatty acid chains in the phospholipid, the membrane will become......

less fluid
more fluid
fluidity will be unchanged

Question 6

Bacteria in a hot spring at 80 degrees celsius would be expected to have ______________ in their cell membrane

high proportions of saturated phospholipids
high proportions of unsaturated phospholipids
an equal ratio of saturated to unsaturated phospholipid

Question 7

Membrane proteins have an important role in membrane fluidity

True
False

Question 8

The HIV virus can't attach to cells that have a mutation in the CCR5 gene and so such individuals are resistant to HIV. What population of people have a higher incidence of the mutant CCR5 gene and why?

europeans because their ancestors survived the bubonic plague
North Americans because their ancestors survived small pox
europeans because they have a longer evolutionary past with HIV
Africans because they have a longer evolutionary past with HIV

Question 9

Nematode trapping fungi have ______________ molecules called ______________ that recognize specific carbohydrates on the surface of the nematode worms. This is how a fungus can recognize that its prey, the nematode worm, has wriggled into the loop. Write two words on lowercase separated by a space with no other syntax

Question 10

Diffusion requires energy

True
False

Question 11

The net direction of molecule movement when molecules diffuse is

down their concentration gradient
random directions

Question 12

In osmosis, water molecules from a solution of higher solute concentration cross a semi-permeable membrane into a solution of lower solute concentration?

True
False

Question 13

Which best defines osmosis?

In osmosis, water from a solution of lower solute concentration move across a semi-permeable membrane into a solution of higher solute concentration?
In osmosis, water from a solution of higher solute concentration move across a semi-permeable membrane into a solution of lower solute concentration?

Question 14

Animal cells placed into a hypotonic solution would most likely?

lyse (burst)
shrink
remain unchanged in size

Question 15

Glucose and amino acids move into cells via carrier proteins, the same protein can move glucose or amino acids

True
False

Question 16

ATP powered pumps move substances into and out of cells by diffusion?

True
False

Question 17

For every 3 Na ions moved out, 2 K ions are moved into a cell by one cycle of the sodium-potassium pump.

True
False

Question 18

Bulk transport of fluid and any of the substances within that fluid is called

pinocytosis
phagocytosis
receptor-mediated endocytosis
exocytosis