PSYC 455 Lecture Notes - Lecture 5: Nicotine, Scatchard Equation
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But, first we need to use a saturation binding curve: Get the concentration of a radio ligand (i. e. any drug/ligand that is tagged with a radio isotope; e. g. , nicotine) by creating samples of increasing concentration of an isotope-tagged drug: The increased concentration gives you a curve (i. e. total binding; e. g. , total binding of nicotine to its receptor). Now we put in a bunch of drug antagonist to saturate the drugs receptors: Subtracting the total binding from the non-specific binding gives us the specific binding (i. e. a curve that levels off; we can only get this curve mathematically): We tend show the specific binding in a more sinusoidal curve (log10 of the concentration). Then, from the specific-binding curve, we look at the concentration (y-value) at which this curve asymptotes/levels off (i. e. this concentration is the bmax; concentration of maximum/no more binding).