CHEM 271 Lecture Notes - Lecture 7: Polyacrylamide Gel Electrophoresis, Two-Dimensional Gel Electrophoresis, Isoelectric Focusing

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For all of the techniques mentioned, you should know what information about proteins and protein structure they provide. You should know the basis for gel electrophoresis. You should know about sds polyacrylamide gel electrophoresis, isoelectric focusing, and 2d gel electrophoresis. You should know what data goes into a purification table, and how to interpret the data in a given table. You should know about the basic procedures for mass spectrometry. You should know about polyclonal and monoclonal antibodies. You should know about green fluorescent protein and some of its uses.