BIOL 467 Lecture 2: lecture 2 - 13-09-2017
Document Summary
Biol 467 13/09/2017 lecture 3 (first 4 minutes are nothing) Model organisms and common experimental techniques in cell biology. Microscopy is an essential tool for cell biology. Light microscopy phase contrast, dic/nomarski, epifluorescence widefield, nearfield tirf, confocal, spinning disc/swept field confocal, multiphoton. Imaging photons: hundreds of nm range, wavelengths impact resolution. Related applications deconvolution, frap, pa, pc, fret, flim, super-resolution storm, Electron microscopy sem, tem, correlative em: detecting electrons, sub-nm range. Airy disk a focused spot of light from a circular aperture determined by the diffraction of light. Picture a dot with a series of concentric rings. How we can resolve that spot of light: that spot of light that is being focused through some sort of circular aperture is going to have a radius. Light, which is waves, will have concentric rings around that spot. If everything is optimal using a widefield epifluorescent microscope you might get a resolution of about 250 nm.
