BIOL 461 Lecture Notes - Lecture 10: Massive Parallel Sequencing, Dihybrid Cross, Genotyping

• School: Concordia University
• Department: Biology
• Course: BIOL 461
• Professor: William Zerges

Document Summary

Using next-generation sequencing to isolate mutant genes from forward genetic screens. Identify mutant gene; sequence the genome with massive parallel sequencing (illumina) Ide(cid:374)tify a (cid:373)utatio(cid:374) that you"(cid:396)e i(cid:374)te(cid:396)esti(cid:374)g i(cid:374) (cid:271)y sequencing the whole genome. Screen in zebra fish (model organism for vertebrates) Can do f3 screens like we saw in drosophila to get mutants that a(cid:396)e affe(cid:272)ted i(cid:374) p(cid:396)o(cid:272)esses that you"(cid:396)e i(cid:374)te(cid:396)ested i(cid:374) Want to identify the gene to get the wt function of the. Wt gene, and you want to get the gene that determines that phenotype. Understand the gene and its product to assign that gene and product to the function. Ems great mutagen because it probes base pairs but hard to find mutation. Next generation sequencing allows you to sequence the genome find the mutation. 1 p(cid:396)o(cid:271)le(cid:373): (cid:449)he(cid:374) you"(cid:396)e just (cid:373)ai(cid:374)tai(cid:374) so(cid:373)e fish/a(cid:374)y o(cid:396)ga(cid:374)is(cid:373), you (cid:272)a(cid:374) (cid:396)a(cid:374)do(cid:373)ly a(cid:272)(cid:395)ui(cid:396)e mutations in the genome (dna polymerase making replication errors, that are not (cid:373)utatio(cid:374)s they"(cid:396)e sile(cid:374)t (cid:373)utatio(cid:374)s(cid:895)