BIOL 371 Lecture Notes - Lecture 13: Green Sulfur Bacteria, Algal Bloom, Enrichment Culture
Document Summary
Biol 371 lecture 13 methods in microbial ecology: exploring microbial abundance, diversity, and activity in the environment. Exploring microbial abundance, diversity, and activity in the environment: culture-dependent analyses. Enrichment and isolation of orgnaisms with specific traits: culture-independent analyses. Environmental genomics (i. e. metagenomics) approaches: measuring microbial activities in nature. Isolation of microbial species: to establish a pure culture of a species that is free of any other contaminating organisms. Enrichment of a microbial species: selection of desired organisms through manipulation of medium and incubation conditions (increased the relative abundance of the organism in relation to the other species) Inoculum: the sample from which microorgnaisms will be enriched or isolated (such as a scoop of soil) Considerations in setting up an enrichment experiment: energy source, electron donors, electorn acceptors, form of macronutrients, form of micronutrients, temperature, salinity, ph, oxygen, a(cid:374)d othe(cid:396) fa(cid:272)to(cid:396)s . The principle of selective enrichment, through an example. The principle of selective enrichment n2 fixers.
