BIOL 261 Lecture Notes - Lecture 7: Stem-Loop, Rho Factor, Consensus Sequence

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It is the link between the message and the amino acids. In eukaryotes dna is in the nucleus, and protein synthesis takes place in the cytoplasm. The link (messenger) between the genes and protein synthesis is mrna. Rna was shown by "pulse-chase" experiments to be synthesized in the nucleus and to move to the cytoplasm. Pulse-chase also showed that rna has a short half-life: rna structure: rna is similar to dna except: Rna polymerase, a dna dependent enzyme, synthesizes rna from the dna template. , ", a, a, s (beta, beta", alpha, alpha, sigma). Beginning and end of the transcription of a gene. Initiation: binding to promoter region -35 and -10 to a "consensus sequence" i. e. a similar, though not identical sequence found in many genes. Elongation: synthesis proceeds in a 5" to 3" direction. Termination: a) gc rich sequence, self complementarity, forming a hairpin loop, followed by a"s: transcription in eukaryotes.

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