BIOL 2303 Lecture Notes - Lecture 11: Metagenomics, Escherichia Coli, Ignicoccus
2 Nov 2020
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Document Summary
A genome is an organism"s full complement of dna, including genes, regulatory elements (those that control transcription and translation) and other elements. Genomics: the study of genomes including: determination of the dna sequences of genomes, gene expression (mrna, protein expression. Identification of genes of potential use in biotechnology (heat stable enzymes: phylogenetics- inferring relationships between organisms. Used to sequence major model organisms: e. coli, yeast, drosophila, human. Next and 3rd generation sequencing- much longer reads. The cost to sequence a genome as decreased significantly with time. The one on the left is the sanger sequencing method and the one on the right is next generation sequencing. Sanger: every time you incorporate a nucleotide that is missing a hydroxyl group, the sequence ends. The computer is then used to detect the colour sequence of the dna to piece them together. Assembly is the process of stitching nucleotide reads together using computational tools.
