BIOL 2303 Lecture Notes - Lecture 6: Pyruvic Acid, Catabolism, Hyperthermophile
2 Nov 2020
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Question: you inoculate a flask of fresh media with 10^6 exponentially growing cells and incubate for 2. 5 hours. You previously calculated the generation time of this bacterium under these growth conditions to be 0. 5 hr. Learning objectives: read cell number values from a log scale graph, describe environmental factors that impact microbial growth, outline the important components of fermentation. Identify the main reactant and products of glycolysis: predict the outcome of perturbations during glycolysis and fermentation. Given a sample, how many cells are in that sample?4: cell counts, turbidity (how cloudy is the sample) Cell suspensions are cloudy because cells scatter light: compare the amount of light that goes through and the amount of light that is absorbed. Cell suspensions are cloudy because cells scatter light. Turbidity is measured by optical density at a particular wavelength using a spectrophotometer. The cells are in the exponential growth phase.
