BIOL 2303 Lecture Notes - Lecture 8: Singlet Oxygen, Rhizobium, Superoxide

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Nitrogen is needed for protein and nucleic acid synthesis. Nitrogen can be obtained from decomposition of proteins or from ammonia, a few bacteria are capable of nitrogen (n2) fixation (rhizobium). Toxic forms of oxygen include : singlet oxygen , superoxide free radicals (o2-) , peroxide anions (o2^2-) and hydroxyl radicals (oh-) = combined name : free. Other chemicals required for microbial growth include sulfur, phosphorus, calcium, etc = combined name: trace elements. A c ulture medium is any material prepared for the growth of bacteria in a lab. Microbes that grow and multiply in or on a culture medium are known as a culture . Agar is a common solidifying agent for a culture medium. (not bacterial food, there to turn liquid medium to solid medium) A chemically defined medium is one in which the exact chemical composition is known. Some parasitic bacteria must be cultures in living animals or in cell cultures.

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