BIOL 2104 Lecture Notes - Lecture 13: Dna Sequencing, Sanger Sequencing, Phosphodiester Bond
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19 Dec 2015
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Lectures 12 and 13: dna structure and replication. The experimental evidence that dna is the hereditary material (details not covered in class but you are expected to know; review in textbook, chap. The structures of the nucleotides of dna (be able to recognize the individual nitrogenous bases, and know which are purines and which are pyrimidines) The mechanism of semi-conservative dna replication including interpretation of meselson- Stahl experiment (details not covered in class but you are expected to know; review in textbook, chap. The reactions carried out by dna polymerases (e. g. e. coli dna polymerases i and iii) Be to able to recognize leading and lagging strands. The roles of the main proteins involved in dna replication, including the various activities needed for synthesizing a complete lagging strand (focus is on e. coli, with the knowledge that fundamentals of dna replication are conserved in all organisms) The main differences between dna replication in prokaryotes vs. eukaryotes.
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