BIOL 3P34 Lecture Notes - Lecture 5: B Cell, Fluorescence, Immunohistochemistry
8 Jan 2018
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Document Summary
Ventricular-subventricular zone stem cell niche and its regulation. Within central region, there are other cell types. Germ cell are connected physically to each other. If vcam blocked, you block the niche and destroy them. Higher level in b cell, more stem cell. Way to visualize gene expression in 3d tissue, Allows to visualize rna expression pattern in whole embryo. Rna is expressed but doesn"t tell you the amount of rna (more blue doesn"t mean more rna: embryo is stained. Odd skipped is recognized as the blue stain because of the combination of enzyme and probe: rna probe complimentary to the region of gene youre interested in. To make a complimentary binding through complimentary bases. Label probe with nucleotides is specific to gen: wash the dna within the embryo. Only odd skip dna will bind to the probe. These pairs will be stable and bathe the embryo in antibody (anti-dig)
