BIOL 2P98 Lecture : Clinical Microbiology and Immunology
16 Dec 2011
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> preliminary and difinitive identification of microbes depends on tests. > immunoflourecence: dyes exposed to uv, violet, or blue light to make flourece. > acid fact, bacteria only (tb & leporacy) > florescent-labled mab target, find out where intruder is located. > if binds after washing you know you have the right one. > expose antibody 2 which is labled to see if if binds. > negative staining stain the background to see cell walls of parasite. > special stains: need to be grown under special conditions ex. intracellular. > sabauraud dextrose agar effective tor most fungi. > cultured within cells ex. embriotic chicken eggs winnow diagnostic test. > looking to hemabsorbtion: binding of red blood cells to infected area. > manual biochemical systems: innoculate wells, watch for results (5-12 hours) > bacteriaphage typing: phage and host cell surface molecules. > ribotyping: clinical way of testing for diseases. > cultural methods unavailable, impractical, or therapy has already started.
