300833 Lecture Notes - Lecture 8: Serial Dilution, Logarithmic Scale, Turbidity
Document Summary
Microbiology revision: viable counting methods, viable counting methods only count those cells that are able to to reproduce when cultured, viable counting methods include spread-plate and pour-plate techniques. The plate count method or spread plate method relies on the bacteria growing a colony on a nutrient medium. Once the colony becomes visible to the naked eyed , the number of colonies on a plate can be counted. Identify similarities and differences between spread plate and pour plate techniques and give examples of their uses(section 7. 7) Spread plate and pour-plate similarities: both plates dilute, a sample of cells before separating them spatially(relating to space). In pour plates the original sample is diluted several times to reduce the microbial population sufficiently to separate colonies when plating. Major sources of error when performing plate counts (mathbench & section 7. 7) Major sources of error in plate counts include: errors in obtaining the sample, errors in loading the neubauer chamber, human errors, dilutions errors.
