300817 Lecture Notes - Lecture 1: Multiple Cloning Site, Expression Vector, Epistasis

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27 Oct 2018
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Researchers in molecular biology use speci c techniques native to molecular biology but increasingly combine these with techniques and ideas from genetics and biochemistry. There is not a de ned line between these disciplines. The gure to the right is a schematic that depicts one possible view of the relationships between the elds: Biochemistry is the study of the chemical substances and vital processes occurring in live organism. Biochemists focus heavily on the role, function, and structure of biomolecules. The study of the chemistry behind biological processes and the synthesis of biologically active molecules are examples of biochemistry: genetics is the study of the effect of genetic differences in organisms. This can often be inferred by the absence of a normal component (e. g. one gene). The study of "mutants" organisms which lack one or more functional components with respect to the so-called wild type" or normal phenotype.

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