BCMB2002 Lecture Notes - Lecture 8: Uncompetitive Inhibitor, Competitive Inhibition, Prostaglandin
14 Jan 2019
School
Department
Course
Professor
Document Summary
Competition between substrate & inhibitor can be biased to favour the reaction by simply adding more substrate. When [s] >> [i], the reaction exhibits a normal vmax. The [s] at which v0 = 0. 5 vmax (apparent km) increases in the presence of i by factor . **mixed inhibition is a type of enzyme inhibition in which the inhibitor may bind to the enzyme whether or not the enzyme has already bound the substrate but has a greater affinity for one state or the other. Uncompetitive & mixed inhibitors: bind to site other than active site. In practice, observed only for enzymes with 2 or more substrates (s1 & s2) If an inhibitor binds to the site normally occupied by s1: act as a competitive inhibitor when [s1] is varied. If an inhibitor binds to the site normally occupied by s2: act as an uncompetitive or mixed inhibitor of s1.
