BIOL214 Lecture Notes - Lecture 3: Acetyl-Coa, Saccharomyces Cerevisiae, Anaerobic Glycolysis

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Understand the essential features of the glycolysis pathway: reduction of nad+, production of atp and pyruvate. Know: reduced form of nad is nadh. Co2 and produce lactate from glucose even under aerobic conditions. glucose gives 2x pyruvate. Glucose = 6c, pyruvate = 3c therefore 1x. Reaction 1: phosphorylation of glucose: phosphate group comes from atp, mg2+ used as cofactor (because p has group allows binding) Reaction 2: conversion of glucose-6-phosphate (g6p) to fructose-6-phosphate (f6p) Reaction 3: phosphorylation of fructose-6-phosphate (f6p) to fructose 1,6. Biphosphate (f16p) different part of molecule: pfk-(cid:1005) has (cid:271)ee(cid:374) (cid:272)alled the (cid:858)gatekeeper(cid:859) of glycolysis, pfk1 catalyses the committed step of glycolytic pathway, proposed to ha(cid:448)e i(cid:373)porta(cid:374)t roles i(cid:374) (cid:373)eta(cid:271)oli(cid:272) reprogra(cid:373)(cid:373)i(cid:374)g i(cid:374) (cid:272)a(cid:374)(cid:272)er(cid:859) Reaction 4: cleavage of fructose 1,6 biphosphate (f16bp: 6c atom converted to 2x 3c atoms. Reaction 5: interconversion of the triose phosphates: can interconvert between forms. Reaction 6: oxidation of glyceraldehyde-3-phosphate (g3p) to 1,3-

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