BABS1201 Lecture Notes - Lecture 17: Dna Profiling, Thermophile, Human Genome Project
9 Aug 2018
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Lecture 17 - 04/05/18 - the polymerase chain reaction. In 2003 human genome project was completed: multiple human genomes have now been fully sequenced, the dna for the first sequence was compiled from several people including james, watson and j. craig venter. Pcr relies on the activity of dna polymerase which requires: dna template (pattern to synthesise on, single stranded dna, primer - short piece of dna to add on to, free nucleotides - to add to the growing chain. Cycles of dna amplification: each cycle doubles number of dna molecules, exponential amplificaion, 2,4,8,16. 32,64,123 . 2^20 is a million, the polymerase chain reaction. Dna polymerase is inactivated at 90 degrees: solution: dna polymerase form the thermophile. Pcr is now conducted in an automated pcr machine: 96 - 382 samples, solid state, programmable, just as every office has a photocopier, evert molecular lab has a pcr machine. Stain: fluorescent under uv light when bound to dna.
