BMS2062 Lecture Notes - Lecture 13: Isoelectric Focusing, Protein Structure, Trypsin
30 May 2018
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Week 7. One gene many proteins
THE NEED FOR PROTEOMICS
• Why do we want to know what proteins are expressed in a cell/tissue?
o Obtain most complete information about components of ells
o Identification of cell type markers
o Identification of disease markers
o Identification of potential drug targets
o Tracking progress of disease or therapy
o Determining presence and effects of protein modifications
o Interactions leading to biological phenotypes
• RNA levels vs protein levels:
o By the time protein expression peaks -> mRNA drops (degraded, has already served its
purpose)
• Lifecycle of a protein:
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Document Summary
The need for proteomics: why do we want to know what proteins are expressed in a cell/tissue, obtain most complete information about components of ells. Identification of potential drug targets: tracking progress of disease or therapy, determining presence and effects of protein modifications. Interactions leading to biological phenotypes: rna levels vs protein levels, by the time protein expression peaks -> mrna drops (degraded, has already served its purpose) Lifecycle of a protein: proteomics- characterisation of all the proteins present in a biological sample including: Identity: chemical form, cellular and sub-cellular location, function. Interactions partners: 3d structure, types of proteomics, expressional (focusing on this) Focused on studying complete proteomes eg. from two differentially treated cell lines. What are their expression levels: functional: studies more limited protein sets to determine their function, structural: high throughput determination of protein structure, expressional proteomics, steps: Determine post-translational modifications: challenges inherent to samples. Dynamic range: challenges in handling and analysis.
