BMS2062 Lecture Notes - Lecture 19: Heteronuclear Single Quantum Coherence Spectroscopy, Oligonucleotide, Proline
30 May 2018
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Week 8. Structure Determination - NMR
PROTEIN STRUCTURE AND INTERACTIONS USING NMR SPECTROSCOPY
• Protein structures can be determined by analysing magnetic resonance data from protein
solutions
• The NMR structure reveals regions of protein flexibility – proteins are breathing and moving in
solution
• Structure determination using X-ray crystallography:
o protein -> crystalline form -> organised array of 3D -> diffraction patterns -> patterns of
spots is collected -> intensity if related to the way X-ray has bounced off electron clouds
on molecules -> can tell you where electron clouds are
o crystal -> data collection -> diffraction -> electron density -> structure
• Structure determination using nuclear magnetic resonance (NMR)
o Do’t eed to rstallise protei
o Have protein dissolved in solution at high concentration -> spots give rise to which
protons are close to each other
o Sample -> magnet -> rise to radio frequency signals -> frequencies of signals can be
visualised
o Method used if ou a’t rstallise protei
o Steps:
1. Express protein – enrich in stable isotypes
2. Optimise sample conditions (high concentration and soluble)
3. Assign signals in spectra (1H, 15N, 13C)
4. Collect spectra to identify pairs of atoms that are close in space
5. Use distance information to calculate family structure – several times to be
confident
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