MIIM20001 Lecture Notes - Lecture 4: Bacterial Growth, Limiting Factor, Doubling Time

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Closed culture vessel (with aluminum; to make sure that we are experimenting with the correct bacteria, no contamination) containing a single batch of fresh medium. Limiting nutrients and increase waste product (no renewal of nutrients and removal of waste) Affect the bacterial growth of the bacteria. Cell are metabolically active but not increasing in number. Synthesizing enzymes, ribosomes or incorporating molecules from the medium. Growth occurs at a constant exponential (logarithmic) rate. Bacteria completing the cell cycle (doubling in number; binary fission) at regular intervals. Doubling time is use as a measure of growth rate. Number of new cells= number of death cells = number of living cells constant. Disadvantages of closed vessels: : when nutrients become limited, waste accumulated and cell population is reached (usually 10^9 million) Conditions in the culture are less supportive of cell division. Counting the number of cells in a defined volume of culture through:

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