GENE20001 Lecture 20: 20 Bacterial Genetics special eukaryotic genetics B

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14 Jul 2018
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At end of second meiotic division we have 4 haploid nuclei. In the next step the nuclei are enclosed. These sacs prevent nuclei from rearranging the order. Meiosis in n. crassa is followed immediately by a single mitotic division producing an ordered octad of spores. The order of the spores in the tetrad reflect the order in which individual chromosomes segregate at meiosis i. Using the gene for albino spores (a-) we can follow the segregation of this gene in a heterozygote transient diploid. Mate one parent that is a+ with one that is a-. Here there was probs no recom between these loci and the centromere. If recombination occurs between a and the centromere. Note that the order has now changed and by looking at the spores in the ascus we can determine that a crossover has occurred in this meiosis. This allows the placement of the centromere on the genetic map of n. crassa.

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