GENE20001 Lecture 19: 19 Bacterial genetics A
Document Summary
Transduction can be used to study dna replication and map the origin of replication (a sequence of dna on which replication machinery assembles) During replication the number of copies of each gene increases from one to two per cell as the replication fork passes each gene. If we could sample the dna at various times after the initiation of dna replication and count the number of copies of each gene then we could identify where replication must have started. If there are twice many copies of the gene is in the cell there is twice the probability the gene will be packaged into a phage in transduction. To map the origin, all of the cells in the culture must be replicating in synchrony. To synchronize dna synthesis in a bacterial culture we use dna synthesis mutants or. Some temperature-sensitive dna synthesis mutants fail to initiate dna synthesis at high temperature.
