GENE20001 Lecture Notes - Lecture 18: P1 Phage, Genome Size, Lysogenic Cycle
Document Summary
Generalised transduction can be used to map genes anywhere on the bacterial chromosome. Specialised transduction is limited to certain regions of the bacterial chromosome. Transduction uses phage to act as a vector carrying genes from the donor bacterium to the recipient. The small genome size of phage means that they can only carry a small portion of the whole bacterial chromosome. Closely linked genes can be separated or mapped by transduction. Temperate but autonomous rather than integrative in the lysogenic state (maintains a separate piece of dna) Occasionally host chromosomal dna instead of phage dna is packaged. A variant of p1 known as p1kc has lost the ability to recognise its own dna and packages host dna at a higher efficiency than the wild type because there is 50 times more host. Wild type is required to produce phage proteins. Infection by a phage particle which has packaged host dna will produce a partial diploid e. coli cell.
