GENE20001 Lecture Notes - Lecture 13: Prophage, Galactose, Repressor
Document Summary
Phage that infect e. coli can be either virulent or temperate. Virulent phage include the "t" series phage: tl, t2, t3, t4 etc. Different phage may carry different forms of genetic material dsdna in t-series and ssdna in m13 and filamentous phage (f1) Can see viruses by the phenotype of the host. If we have a plate we grow a lawn of e. coli on it. These are areas where the bacteriophage have lysed the bacteria. Circularisation of phage dna; recruitment of host machinery. Genes on dna are expressed to produce phage components. Assembly of phage from components and packaging of dna to form a mature phage. Lysis: release of progeny phage (>100), which go on to infect neighbouring bacterial cells. They produce enzyme that weakens cell wall of bacteria. All the progeny are clones (unless they mutate) Plaque: area on a bacterial lawn where all cells have been lysed, leaving a cleared area.
