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BIOL10005 Lecture 1: BIOL10005 Lecture 1 Notes

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ceriseeagle929
20 Sep 2018
School
University of Melbourne
Department
Biology
Course
BIOL10005
Professor
Dawn Gleeson

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Document Summary

Epigenetics: changes in gene expression without changes in the dna sequence. Gene: the fundamental physical and functional unit of heredity, which carries information from one generation to the next, a segment of dna. Genetics: the study of the variation between and among living things, and how this variation is inherited. Genome: the total amount of genetic material in a single set of chromosomes stored as dna sequences, sequencing a genome is working out base sequence of dna. Genotype: the speci c allelic constitution of an organism, either of the entire organism or for a certain set of genes or a certain gene, comes from parents. Phenotype: the morphological, biochemical and behavioural properties of an organism resulting from a speci c genotype and its interaction with the environment. Control or in uence nearly all characteristics or phenotypes of an individual. Genomes (not genotype) can change throughout the life of the organism. Related organisms have similarities in their genome sequences.

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Related Questions

Expression vectors inprokaryotes do not make functional eukaryotic gene products inbacteria very well because

Answer Not sure which one ?!

· the codon sequence for prokaryotes is differentthan the codon sequence in eukaryotes

· there are no disulfide bridges formed in proteinsnormally made in prokaryotes

· prokaryotic expression vectors cannot translateeukarytic sequences

· eukaryotic genes have introns, and prokaryotesdon't

· eukaryotic genes have exons and prokaryotesdon't


I create a knockout mouseusing the agouti/black fur system \. When I cross the agoutioffspring of the originally engineered mouse, I find a ratio of 2agouti mice to 1 black furred mouse. What is the bestexplanation?

Answer not sure which one?!

· The gene knocked out was recessive.

· The gene knocked out was dominant.

· The gene knocked out was a lethalgene.

· The knockput was integrated into a random spot,and did not knockput the original gene.

A restriction enzyme cuts DNAand leaves the following end

xxCTGCA
xxG

Which of the following could be the sequence of the correspondingend of the other end of the cut DNA?

Answer

· xxG
xxCTGCA

· xxC
xxGTGCA

· xxCCGAT
xxG

· xxGGCTA
xxC


An SNP always occurs dueto

Answer

· a one base-pair change.

· a three base-pair change.

· a single restriction site change.

· a single amino acid change.

· None of these.


I PCR out a mutated gene in apatient with Lisenbee chorea (the inability to dance in acoordinated fashion) and I compare it to another patient with thatsame disease phenotype. One subject had a mutation on chromosome 4,and the other subject couldn't rock it because of a mutation onchromosome 5. This is an exampe of

Answer

· pleitropy

· locus heterogeneity

· allelic heterogeneity

· dominant negative mutation

· penetrance

In his experiments, Mendelnoted that when two traits are involved in a genetic cross, theyare inherited independently of each other. Though Mendel didn'tknow about chromosomes, this still holds true (mostly)because

Answer

· genes on the same chromosome are often mixedthrough meiotic crossover during the formation ofgametes.

· variant alleles of the same gene do not interferewith the phenotypic expression of the gentoype in different geneticloci.

· most higher organisms are diploid, making only twoalleles available for every genetic locus, and there is a 50/50chance of geting either allele from a single parent.

· expression of phenotypes can vary from thegenotypes because of encironmantal influences.

· chromosomes often recombine

Anticipation is caused by amutation that increases in expressivity over subsequentgenerations.

Answer

· True

· False

Question 44

I have a genotype that shouldproduce a specific phenotype, but some of the individuals with thegenotype do not demonstrate any evidence of the phenotype. I wouldconsider this an example of

Answer

· allelic heterogeneity.

· locus heterogeneity.

· penetrance.

· expressitivity.

· sheer luck.

Question

Which of the following doesnot occur during the PCR reactions?

Answer

· Changes in the reaction temperature by athermocycler

· Denaturation at high temperatures

· Synthesis of oligonucleotide primers

· Extension of primers by DNA polymerase

· Annealing of oligonucleotide primers

Question

DNA markers, or variantnon-coding regions of DNA, are DNA polymorphisms that are usefulfor genetic mapping.

Answer

· True

· False

A mosaic is an organismwith

Answer

· multiple genotypes within one organism

· multiple alleles within one genotype

· more than one color of fur

· transgenes added to the zygote beforedevelopement

· a wt phenotype but a mutated genotype

Question

Genotype causesphenotype.

Answer

· No, gentoype just influences phenotype.

· Yes, genotype is the DNA sequence that createsphenotype.

Question

A true genetic chimera can becreated by

Answer

· mutating a gene early on in the development of anorganism resulting in different alleles being present in theadult

· multiple fertilized eggs or zygotes fusing to formone embryo

· adding a transgene to the genome of an organismduring fetal development only

· adding cells of a different species to an adultorganism

· adding a transgene to the genome of an animal atany stage in development

Question

The ABO blood group can bestbe explained by the concept of _______.

Answer

· dominant traits

· recessive traits

· allelic heterogeneity

· locus heterogeneity

· vampirism

If a loss of functionmutation creates a dominant phenotype, it may be becauseof

Answer

· haploinsufficiency

· penetrance

· expressivity

· allelic heterogeneity

· locus heterogeneity

Please select the best matchfor each.

Answer

· PCR: Technique used to generatecopies of a DNA fragment

· Southern blot : Screeningprocedure used to assay DNA fragments

· RFLPs : DNA fragments generatedby restriction enzymes that vary in length betweenindividuals

· Northern blot : Screeningprocedure used to assay mRNA fragments

· Electrophoresis Movement ofmacromolecules in an electrical field

Answer

A.

Screening procedure used toassay DNA fragments

B.

Movement ofmacromolecules in an electrical field

C.

Screening procedure used toassay mRNA fragments

D.

DNA fragments generated byrestriction enzymes that vary in length betweenindividuals

E.

Technique used to generatecopies of a DNA fragment

A gene mutates, and theprotein produced has a novel way of interacting with the cell, andcreates a new phenotype because of this new functionality. This iscalled

Answer

· pleitropy

· locus heterogeneity

· allelic heterogeneity

· dominant negative mutation

· gain of function dominant mutation

Question 62

Mutations in the somaticchromosomes may be inherited by the next generation.

Answer

· True

· False


A degenerate PCR primer withmany variant sequences must be used to make multiple copies of agene

Answer

· if only the protein sequence of the gene productis known to construct the primers

· if the DNA you are using is cDNA to constructthe primers

· if the DNA you are using is genomic DNA to becopied

· if the DNA you are trying to copy iscDNA

· if the vector is prokaryotic and the transformedcell is eukarytotic

Question

Please select the best matchfor each term.

Answer

· Restriction enzyme


· PCR C

· Plasmid

· YAC A

· Dideoxynucleotide

Answer

A.

Endonuclease isolated frombacteria

B.

Eukaryotic cloningvector

C.

Method for amplification ofDNA sequences

D.

Small circular fragment ofDNA

E.

Molecule used in DNAsequencing

Question

A couple goes on MauryPovitch, and the results are in: you are not the father. But noother man impregnated the female (granted, unlikely for a MauryPovitch guest, but work with me here) and he must be the father.DNA analysis claims otherwise, though the child definitely wasmom's (poor thing). What may be going on here?

Answer

· the child is parthenogenic because the motheractually impregnated herself like a shark, and the child's DNA isall mom's

· the child had a mutation that changed the genethat is used to trace paternity

· the child is a mosaic because he is actually a setof twins fused early during fetal development, and therefore camefrom 2 eggs and 2 sperm

· the dad may have germ-line mosaicism, meaning thatthe genotype of his sperm is different from his somaticgenotype

· mitochondrial DNA only comes from mom, so there isno way to tell whobthe baby's father is




QUESTION 10

If you can drink milk as an adult, it means that you have inherited a mutation in the promoter of your lactase gene (the gene that encodes the enzyme you need to break down lactose). Predict the effect of this mutation:

The mutation changes the number of domains in the enzyme, which makes it work more efficiently

The mutation changes the amino acid sequence of the lactase protein

The mutation increases the number of copies of the lactase gene that will be found in your genome

The mutation changes whether the lactase sequence is found in an intron or exon

The mutation affects the expression of the lactase gene

1.2 points

QUESTION 11

A competitive inhibitor is decreasing the activity of an enzyme. Predict the effect of adding more substrate to the reaction.

The substrate will increase the reaction rate by binding to the allosteric site

The substrate will increase the reaction rate by competing with the inhibitor for the active site

The reaction rate will not change unless the inhibitor can be removed

The enzyme adjusts its shape so that the substrate, but NOT the competitive inhibitor, can bind

The substrate will bind to the competitive inhibitor and block its ability to bind to the enzyme

1.2 points

QUESTION 12

What determines where in the genome a transcription regulator will bind?

Transcription regulators bind to the 5' UTR region of a gene

Regulators bind via complementary base-pairing to certain DNA molecules

Covalent bonds form between the transcription regulator and the atoms of the DNA backbone

Every eukaryotic gene has a different transcription regulator that will bind to the 5' end of the gene

Transcription regulators bind to specific DNA sequences via multiple weak non-covalent interactions

1.2 points

QUESTION 13

What is the basic premise of cell theory?

DNA -> RNA -> protein

All cells arise from pre-existing cells

DNA provides the complete instructions to create a cell

The identity of a cell is determined through gene expression patterns

All cells contain the same four basic macromolecules

1.2 points

QUESTION 14

What is the benefit of using BOTH the lac activator and the lac repressor to control gene expression?

Using both an activator and repressor enables cells to more accurately determine the amount of lactose available in the environment

Enzymes to digest lactose are only made when energy is low and lactose is available

The activator can override the inhibition of the lac operon by the repressor

The repressor can control the enhancer, while the activator can control the promoter

When neither the lac activator or repressor is present, expression of the lac operon is too high

1.2 points

QUESTION 15

What is the histone code used for?

Phosphorylation and acetylation of DNA affect its ability to be compacted

Changes to the sequence of DNA change whether DNA will wrap around histone proteins

Covalent modifications of histones affect the ability of the transcription initiation complex to form

Histones provide the codon sequences needed for translation to occur

The histone code affects which amino acids will get added to a polypeptide

Ploidy refers to the number of complete sets of chromosomes (genes) present in a cell. The number of chromosomes in a complete set is a fixed characteristic of a species. Humans are diploid with two sets of 23 chromosomes. Fruit flies are diploid with two sets of 4 chromosomes. Sexually reproducing organisms are diploid, each somatic cell of an organism has two complete sets ("di" is a prefix indicating 2.). To complete a life cycle and reproduce new members of a species, mating is required. Mating brings a donation from a male and a donation from a female together. However, if the male and female cells remain diploid, the fertilization will result in a tetraploid zygote. In general, these are not viable. A mechanism is needed to form special haploid cells, gametes. The diploid set is split precisely into two complete haploid sets. That mechanism is meiosis.

The complete set of chromosomes of a species is also the complete set of genes of a species, the genome. (Yes, there is a minor contribution from mitochondria and chloroplasts. Inheritance of this information is "cytoplasmic".)

Please answer the following questions:

1. How many chromosomes in a brain cell of a fruit fly? How many chromosomes are in a liver cell of a human? Explain both answers.

2. How many chromosomes are in a fruit fly unfertilized egg? How many chromosomes are in a human egg? Explain both answers.

3. Why is meiosis necessary for sexually reproducing organisms? How many chromosomes are in a human zygote? Relate the two answers.

4. What is a gene allele? Suppose there is a gene called "bingo" and it is present once in a complete haploid set. How many alleles of "bingo" does a diploid individual have? How many copies or versions of the gene "bingo" are present in a diploid individual?

5. Modern DNA sequencing clearly identifies alleles by showing the different DNA sequence of each allele. These alleles may be termed structural alleles (I coined the term for this problem.). Classical genetics only dealt with phenotypes or visible traits. As a result, alleles of genes were identified by functionality, usually normal or non-functional. Using your knowledge of gene structure and gene expression, postulate a relationship between structural alleles and functional alleles. (include relative amounts; more, less, or equal).