BCMB20005 Lecture Notes - Lecture 6: Chlorocebus, Jonas Salk, Protein Purification

• School: University of Melbourne
• Department: Biochemistry and Molecular Biology
• Course: BCMB20005
• Professor: Amber Willems

Document Summary

All figures from lecture 6a slides, a willems-jones, march 2017, melbourne university (unless stated) Tissue culture technique: types of cells, how cells and their structures can be imaged. Scientific discipline of studying cells including protozoa, bacteria and eukaryotes, at a cellular level (in vivo. Active and passive transport movement of molecules into and out of cells. Cell signalling pathway regulation of cell behaviour by signals from outside: lots of many pathways, many of the molecules have ligands that bind to a receptor molecule, and activate a transcription factor in the cytosol. This in turn starts a translocation to the nucleus, where activation/repression of target genes: sometimes cancer leads to mutations in receptors, e. g. the tgf receptor, and leads to cellular proliferation, or invasion. Hence the opposite to the receptor"s job happens. To be able to target proteins for their functions: such as those that bind to cells, work in different pathways.