BM1000 Lecture Notes - Lecture 27: Asepsis, Cell Division, Turbidity
Document Summary
How knowledge of microbes biosynthetic capacity relates to laboratory culture. Organisms must be isolated and cultivated to be identified. Using broth cultures, we cannot tell what is growing. Need individual colonies which can be visually compared. Liquid at 100dc, solid at ~40dc tures". ally. Use knowledge of microbial nutrition to create artificial systems. Culture media: nutritional solutions used to grow microbes in a lab. Precise amounts of pure chemicals added to water. The time taken for one cell to become two. Growth does not begin immediately when organisms are placed in culture media. May require synthesis or enzymes for production of metabolites not provided. Influenced by composition of media, temperature, organism specific characteristics. When the above factors over-ride the ability for cells to remain viable. ics. Growth measured by either direct or indirect methods. Many bacteria have never been grown in culture medium.
