BIOL123 Lecture Notes - Lecture 9: Focal Length, Tungsten, Plasmodium

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17 Jun 2018

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• Light sources:

• Illumination needs to be bright, glare-free, evenly dispersed

(tungsten, fluorescent , LED, quartz-halogen)

• Structure (distribution of light and source geometry), wavelength

distribution, spatial and temporal stability, brightness and degree

to which all these can be controlled

• Focal length, magnification, aperture of collection lens

• Light-gathering power of the objective (governs image

brightness)

• Specimen illumination:

• Best conditions are: opaque (viewed by reflected light/ epi-

illumination) or transparent/translucent (viewed by transmitted

light)

2.6 Understand and recognise types of objectives for light microscopes and

their use:

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