MICROM 301 Chapter Notes - Chapter 18.4: Primary And Secondary Antibodies, Radionuclide, Microscope Slide
Document Summary
18. 4 common types of immunoassays: antibodies are small molecules that cannot be seen using conventional microscopy, thus developed assays to monitor antigen-antibody interactions, assays fall into two general categories. Immunoassays that involve antigen antibody aggregates: not used as much now, relatively inexpensive and simple, some still useful in certain situations. Immunoassays that use labeled antibodies: basic principles, direct immunoassays, used to identify unknown antigen i. e. certain pathogen in a clinical specimen. If label not detected, specimen did not contain antigen x b) c) Indirect immunoassays: used to detect antibodies of given specificity in patients serum. Indirect because they require a labeled secondary antibody to b) detect unlabeled first antibody. I. e can be used to characterize different types and stages of lymphomas. 2. and leukemias as well as to diagnose immunodeficiency disease: can also be used to track progression of hiv disease by determining serum levels of cd4 t cells.
