BIOL 4246 Chapter : 6 13 12 Initiation Of Transcription
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Lower mgcl concentration and higher the temperature the more specific the annealing. A good yield of the single product you are after. Rna as a macromolecule snrna rrna both function by bp complementarity ribose switche4s on the 5" untranslated region of an mrna that regulate the mrna"s expression. 3 basic ways that this functions- autocleaving rna- no longer functional. Block translation by preventing ribosome from finding rna. Small rnas can also do this as well. Rna polymerase subunit structure, differs a little bit from archaea. Rna poly subunit strucutre- e. coli as an example: large multisubunit enzyme where each subunit contributes somewhat to the enzyme, subunits, beta prime- 1407aa (110 da/aa)= 155,000 da. Function in the rna polymerase is dna binding. ssdna and dsdna. Has part of the helicase activity (1/2 of the domain): beta- 1342 aa (amino acids) so around 150,000 da. Has the catalytic site that synthesizes the rna.
