BIOL 4246 Chapter : 7 2 12 4246
Document Summary
Ruvab has an essential function in dna repair, not dna exchange. If you mutate ruvc rusa will have a role. Transmission- two types of transposons that use transposase using diferent modes. Is uses cut and paste mechanism, conservative mechanism. Complex go through replicative mechanism, transposase cuts two strands, not all the way out, the whole complex binds and forms an intermediate in which two dna molecules are using one transposon. Transposition mechanisms/ transposons: short version of site-specific recombination, catalyzes gene exchange between two molecules, each containing one of its recognition sites, outcome depends on the orientation of the recognition sequences. Mechanism is a 2 step process using a holiday junction intermediate followed by resolution. Make a tyrosine phosphate bond when you reform a new strand: s-serine recombinase, rebond with a serine phosphate, use a 4 strand synaptic complex. All 4 strands involved in the recombination in 1 step: super-integrons, superpathogens- vibrio cholera- can have a number of cassettes.
