BIOL 2153 Chapter : Ppts Typed
Document Summary
Robert hooke: frist to describe microorganisms, 1665. Antoni van leeuwenhoek: first to describe bacteria, 1676. 4 types of light microscopy: brightfield, simplest, specimen illuminated, magnified 1000x, specimen stained to increase contrast, phase contrast and darkfield, visualize live samples, no staining required, fluorescent. Compound light microscopes: brightfield, phase-contrast, darkfield, fluorescent, optimize image resolution by using lenses with high light gathering charateristics, limit of resolution 0. 2 um. Electron microscopes: limit of resolution 0. 2 - 4 nm, two types of electron microscopy, transmission electron microscope (tem) for observing internal cell structure, scanning election microscope (sem) for three dimensional imaging and viewing surfaces. Basic components of all microbial cells: cytoplasm, cytoplasmic membrane, ribosomes. Two structural types of cells are recognized: prokaryote and eukaryote. Prokaryotic cell: cell wall, cytoplasmic membrane, cytoplasm, plasmid, ribosomes, nucleoid. Eukaryotic cell: cell wall, cytoplasmic membrane, endoplasmic reticulum, ribosomes, nucleus, nucleolus, nuclear membrane, golgi, cytoplasm, mitochondrion, chloroplast (some photosynthetic cells)
