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BE 2352 Chapter : BE 2352 Lab4

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azuremammoth471
15 Mar 2019
School
LSU
Department
Biological Engineering
Course
BE 2352
Professor
All

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Document Summary

We will perform this lab to determine what effect two different water temperatures (room temperature and warm) have on the rate of reaction of a caplet of sodium bicarbonate. Also, we are setting out to determine whether or not the. The rule of thumb states that when increasing the temperature by 10 degrees celsius, the reaction rate doubles. In this experiment, the mass of a tablet is determine, then mass of water in a cup is determined, these numbers are all added together to be the initial mass. Then the mass is determined every five seconds for a minute after being placed in either warm or room temperature water. The first reaction (using cold water) is a zero-order reaction. We are able to tell it is a zero-order reaction since the line is practically straight. This means the reaction is independent of the concentration of the reactant. The second reaction (using warm water) is a first-order reaction.

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Related Questions

A 5 00μl reaction co ntaining 0.5 pmol of an enzyme following Michaelis – Menten kinetics with saturating substrate concentration is studied . For this enzyme k1 = 1 x 10 9 M - 1 s - 1 , k - 1 = 2 x 10 3 s - 1 , and k2 = 1 x 10 5 s - 1 .

a . What is the Vmax for the enzyme considering that it has only one substrate binding site ? b. What are the values of Km and Ks? Is this a rapid equilibrium enzyme or does it follow steady state kinetics? Explain why .

c . What is the substrate concentration needed to achieve half maximal velocity? Explain the basis for your answer.

d . If the substrate concentration in part c is doubled , how many fold will the initial velocity increase?

e . What is the Km i f the concentration of the enzyme is doubled? f . What is the efficiency of this enzyme?

g. Would this enzyme be considered a perfect enzyme? Explain.

h . If 0.25 pmol of an irreversible or classic non - competitive inhibitor is added before starting the reaction. What is the resulting Km for the enzyme?

Lab 1 – The digestive system and food safety

Scenario/Summary

One of the first topics that we need to explore is our digestive tract. Each step of digestion has its own function. Chewing, for example, increases the surface area of food particles (physical digestion) so that the enzymes in the mouth can start breaking the nutrients down (chemical digestion). Review the two links and complete the lab assignment stated below.

Deliverables

First examine the interactive links provided and then follow the steps below. The assignment should

be two pages maximum, double spaced, 12-point font, and have one-inch margins with standard

APA referencing. Lab reports are to be written in Microsoft Word and submitted for grading.

Lab Steps

Part 1

There are several parts to this Lab. Part 1 consists of listing all of the parts of the digestive system with either a list or photo. Many people take a screenshot and use it for Part 1. Feel free to also list the parts as well if you cannot figure out how to take a screenshot.

Part 2

Part 2 technically consists of two parts. The first one includes describing the location of each body part. For example, the appendix is located on the right side of the body under the ascending colon. Another way to do this part would be to write out the parts and put a location/description next to it. The second part of Part 2 for this section is to describe the manner in which you will retain the information of where each body part is located. An example would be, "I will remember the location of each body part by reviewing the photo and memorizing each location." Some classmates develop interesting methods of memorizing where all of the parts are located, like using mnemonics. Feel free to be creative.

Cell Membranes Lab Report

  1. Diffusion

  1. Compare the following terms: solvent, solute, andsolution.

  1. Go to Lab, Section I, Exercise 1 to explore the factorsinfluencing the rate of diffusion and complete the following:

  1. Predict how molecular mass and temperature may affect theoutcome of this experiment.

  1. Record the data from information in the Lab in the tablebelow.

Time

(min)

Diameter (mm)

5°C MB

5°C PP

23°CMB

23°C PP

0

15

30

45
  1. Construct a line graph of the data from the table above. Yourgraph should include labeled axes, units, and a legend indicatingwhich treatment is presented. Sign, date and prepare an image ofyour graph and include it with this lab report.
  1. Osmosis

  1. Go to Lab, Section II, Exercise 2 to view a demonstration ofosmosis and answer the following questions:

  1. Describe the net movement of water in osmometer 1.
  1. How is the movement of water molecules related to theconcentration gradient of the solution?

  1. Go to Lab, Section II, Exercise 3 to observe the effect ofsolute concentration on the rate of osmosis. Answer the followingquestions:

  1. Was the net movement of water in bags 1 to 4 into or out of thebags?
  1. Explain the results from bags 4 and 5.
  1. Selective Permeability of Membranes

  1. Go to Lab, Section III, Exercise 4 to learn about theimportance of selectively permeable membranes, then complete thetable and answer the following questions:

Contents of Beaker

0 Min

30 Min

Starch

Chloride ion
  1. Which substances diffused through the dialysis membrane?

  1. How does dialysis tubing model the selective permeability of aplasma membrane?
  1. Tonicity

  1. Go to Lab, Section IV, Exercise 5 and 6 to observe plasmolysisin Elodea cells and tonicity in red blood cells. Summarizethe concept of tonicity using blood and Elodea cells asexamples. Your description should incorporate these terms: turgid,plasmolysis, hemolysis, and crenation.

Cells

Isotonic Solution

Hypertonic Solution

Hypotonic Solution

Elodea

RBC

  1. Based on your previous work, reproduce the table below and drawan illustration of a single cell in each box. Include arrows toshow the direction of water flow relative to each cell and dots tosymbolize solutes. Sign, date and prepare an image of your drawingand include it with this lab report.

Cells

Isotonic Solution

Hypertonic Solution

Hypotonic Solution

Elodea

RBC

Summary Questions

  1. Compare diffusion and osmosis. Give an example of each.

2. In which direction will osmosisoccur if a 15% sugar solution is separated from a 25% sugarsolution by a selectively permeable membrane?

3. Why did osmosis, but not diffusionof sucrose molecules, occur across the dialysis membrane containing20% sucrose solution?

4. You are having a party and thecelery is limp. What might you do to make the celery crisp (turgid)again? What will occur in the cells?

5. A small amount of fertilizer(mineral salts) will stimulate plant growth, but over fertilizationcan kill plants? Why?

6. Michael adds sugar to his coffee.Explain what in this drink is the solvent, solute, and solution.

7. You add a cube of sugar to yourdrink. How could you speed up the diffusion of sugar moleculeswithout stirring?

8. How is dynamic equilibriumestablished within a solution?