CAS BI 107 Chapter Notes - Chapter 20: Sanger Sequencing, Insecticide, Rna Biology
Document Summary
Recent advances in the tools used by biologist have enabled them to investigate new areas of biology leading to breakthroughs in drug development, genetic engineering, and understanding of the world around us. Concept 20. 1 dna sequencing and dna cloning are valuable tools for genetic engineering and biological inquiry. Understanding the chemistry of dna and its properties has enabled the development of tools to manipulate sections of dna and replicate them as desired (dna cloning) and to readout the sequence of bases (dna sequencing). Dna sequence information is commonly obtained using 2 methods: chain termination or sanger sequencing (invented by dr. frederick sanger). Dna to be sequenced is polymerized using a primer piece of dna at a known site and a mixture of fluorescently labeled ddntps and unlabeled dntps. Synthesize of dna extends until a ddntp is incorporated. These fluorescently labeled pieces of dna are resolved by running through a gel which separates them based on size.
