MICB 201 Chapter Notes - Chapter 6-7: Genome Size, Inverted Repeat, Proteome

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15 Dec 2017
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In a genome, the location of the gene is fixed. Different coloured corn kernels are linked to mobile genes called transposons. Carry other genes as well (ab resistance, cause infection, metabolism) Non-replicative: moves within the same molecule (doesn"t involve copying itself) Replicative: moves between two dna molecules in the same cell (makes a copy of itself) On the slide, the red boxes represent dsdna. Help identify the tn to the enzyme or protein that is doing the moving. Donor location: where the tn starts out (where the process begins) Tpase proteins recognizes the target site between recipient location specific nucleotide sequence (different for different tns) can be located anywhere can be problematic if located into a gene encoding site. Tpase has endonuclease activity and can transport tn to new location. i. e. breaks sugar phosphate backbone (on different sides), inserts into target site, then catalyzes phosphodiester linkages. Everything else between replicative and nonreplicative is the same.

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