KIN217 Chapter Notes - Chapter 18: Pyruvate Dehydrogenase Kinase, Cardiac Output, Acidosis

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Citric acid cycle oxidizes 2-c units (acetyl coa), producing 2 co2, 1 atp/gtp, and high-transfer- potential electrons. 4-c acceptor molecule that accepts the acetyl coa is regenerated at the end to continue the cycle. 18. 1 pyruvate dehydrogenase forms acetyl coenzyme a from pyruvate. Produces co2 and captures high-transfer-potential electrons in the form of nadh large, highly integrated complex of 3 distinct enzymes, each w/ own active site. Dihydrolipoyl transacetylase (e2) transfer of acetyl group to coa. Dihydrolipoyl dehydrogenase (e3) regeneration of oxidized form of lipoamide (prosthetic group for e2) Synthesis of acetyl coenzyme a from pyruvate requires 3 enzymes and 5 coenzymes. Catalytic coenzymes are not permanently altered; stoichiometric coenzymes function as substrates. Conversion of pyruvate into acetyl coa consists of 3 steps: Transfer of resultant acetyl group to coa. These steps must be coupled to preserve the free energy derived from the decarboxylation step to drive the formation of nadh and acetyl coa.

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