CHEM237L Chapter Notes - Chapter 5: Alkaline Phosphatase, Cuvette, Enzyme Kinetics
12 Jul 2017
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Understand steady state enzyme kinetics and reactions, analyze enzyme inhibition interactions, be familiar with kinetic parameter plots, and find. 1: read kinetic studies with alkaline phosphatase in the presence and absence of inhibitors and divalent cations dean rl. Alkaline phosphatase, enzyme buffer, assay buffer, substrate pnpp, substrate with inhibitor (pnpp + pi), 10 cuvettes, 7 microfuge tubes, cuvettes, pipette tips, eppindorf. Set up alkaline phosphatase activity assays [substrate = 200um pnpp] 410nmm 0. 1 (s), xmode = min, y: 0-0. 2 (min/max), collect = simple, cycle = 0. 02min, stop = 1min, options: select results/graph/user data form, storage on. Zero with 1ml of assay buffer then rinse it out. 4 have: cuvettes 1-7 filled with 100ul ap, blue eppindorf to 1000, box of tips, 10ml of 200um pnpp in test tube. Start > save as c254 (record computer id and file name) 2min countdown starts.
