BIOL 205 Chapter Notes - Chapter 10: Werner Arber, Molecular Cloning, Recombinant Dna
13 May 2017
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Document Summary
The first step to studying gene function is to isolate its dna and reproduce it in quantities suitable for study. One way to separate our gene of interest from the rest of the genome is to cut the genome with molecular scissors and isolate a small fragment containing the gene. Werner arber discovered that resistant bacteria possess a previously unknown enzyme: a restriction endonuclease that cuts dna at specific sequences, the enzyme he discovered, ecori, became the first commercially available molecular scissors. Dna technology describes the collective techniques for obtaining, amplifying and manipulating specific dna fragments. Genetic engineering is the application of dna technology to specific biological, medical or agricultural problems. Genomics is the ultimate extension of the technology to the global analysis of nucleic acids present in a nucleus, cell, organism or related species. 10. 1 overview: isolating and amplifying specific dna fragments.
